Director: Prof. Dr. Dr. D. Schild
PoissonNMF is a ImageJ plugin that allows to decompose lambda stacks of fluorescently labeled samples into the contribution of the individual dyes, even if the spectra of the dyes are not or only approximately known. The method is described in Neher et al., Biophysical Journal. In addition to blind unmixing, the plugin can also be used for standard or non-negative unmixing.
HTML documentation
Tutorial using an example with three dyes imaged on Zeiss LSM meta 510
Richard Neher
Fabian Theis
Andre Zeug
Please address comments and questions to Richard Neher and notify us of any bugs you find.
ImageJ: rsbweb.nih.gov/ij
Linear unmixing plugin by Joachim Walter: Spectral Unmixing Plugins
2009/02/25 (version 0.8.7)
Implemented the data visualization feature "Simplex projection".
2009/02/18 (version 0.8.6)
First release.