Methods used in the lab
Two photon excitation microscopy
Axons entering the lateral part of the OB.
Especially in thick tissue samples, confocal laser-scanning microscopy is limited by light scattering. Two photon excitation only excites fluophores in the focal plane without the necessity of a pinhole to limit the emitted light. The localization of excitation is the major advantage in comparison to cLSM. In addition, the longer wavelength excitation light permits deeper tissue penetration. Altogether, this allows the detection of fluorescent signals in thick tissue slices and even in whole mount preparations.